Useful Links & Tools
- MSDS
- Specification Sheet
- Certificate of Analysis
- Certificate of Origin
- H3784 - Product Information Sheet (28 KB)
- HEPES Sodium Salt Specification Chart (117 KB )
- FT-IR Raman
- FT-IR Condensed Phase
H3784
Sigma
HEPES sodium salt
Biotechnology Performance Certified, cell culture tested, ≥99.5% (titration)
| Synonym: | 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt |
| CAS Number: | 75277-39-3 |
| Linear Formula: | C8H17N2NaO4S |
| Molecular Weight: | 260.29 |
| EC Number: | 278-169-7 |
| MDL number: | MFCD00036463 |
| PubChem Substance ID: | 24895595 |
Description
| Physical form | Lyophilized, spray dried, or crystallized material. |
Properties
| grade | Biotechnology Performance Certified |
| assay | ≥99.5% (titration) |
| total impurities | Endotoxin and bioburden, tested |
| useful pH range | 6.8 - 8.2 |
| pKa (25 °C) | 7.5 |
| EQP level | Elite |
| cation traces | Fe: ≤5 ppm |
| heavy metals (as Pb): ≤5 ppm | |
| abs. | A |
| A | |
| suitability | cell culture tested |
| foreign activity | DNase, RNase, protease, none detected |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| Safety Statements | 22-24/25 |
| WGK Germany | 3 |







